Growth, pigments production and phytochemicals variation in Tetraselmis chui under different cultivation parameters

Document Type : Original research

Authors

• Norhayati Yusuf
• Nurul Shafiqa Yusof
• Malinna Jusoh
• Hazlina Ahmad Zakeri
• Nurfarha Muhamad Zalan

Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

10.22034/iar.2023.1993193.1481

Abstract

This study investigates the impact of different cultivation conditions on Tetraselmis chui, a microalgae species with vast potential in renewable energy, pharmaceuticals, and nutraceuticals. Various parameters, including media strength (F, F/2, F/4, and F/8), sucrose concentration (0-50 g/L), medium pH (5-9), and light intensity (0-600 lux), were examined. The study focused on growth, pigment content, and phytochemical composition, which is crucial for its commercial value. After eight days of cultivation in F2 media, T. chui exhibited typical growth phases, with full-strength media being the most effective in promoting cell growth. The addition of sucrose positively affected cell growth, especially at 40 g/L. The pH of the medium did not significantly impact growth, except at pH 5, where growth was affected negatively. Higher light intensities gradually increased cells' fresh and dry weights. Furthermore, chlorophylls a, b and carotenoids accumulated more in full-strength media supplemented with higher sucrose concentrations and under higher light intensities. Lower pH levels inhibited the synthesis of these pigments. Additionally, total phenolic compounds and flavonoid production were higher in a full-strength medium supplemented with higher sucrose concentrations, light intensities, and pH levels. These findings have significant implications for the development of sustainable microalgae cultivation strategies. By optimising cultivation parameters, including media composition, sucrose concentration, light intensity, and pH levels, the production of desired compounds and biomass can be enhanced, contributing to the advancement and sustainability of various sectors.

Keywords

• Tetraselmis chui
• Growth
• Pigments
• Phytochemicals
• Cultivation parameters

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