Document Type : Original research
Authors
1
BEMAREMA´s Doctorate, Coast University Centre from Guadalajara University. Puerto Vallarta, Jalisco, Mexico
2
Biomedical Research Centre, Autonomous Campeche University. San Francisco de Campeche, Campeche, Mexico
3
Biological Science Department, Coast University Centre from Guadalajara University. Puerto Vallarta, Jalisco, Mexico
4
Ecology and Fisheries Laboratory, EPOMEX Institute, Autonomous Campeche University. San Francisco de Campeche, Campeche, Mexico
5
Agricultural Science Faculty, Autonomous Campeche University, Escarcega, Campeche, Mexico
6
Molecular Microbiology Laboratory, EPOMEX Institute, Autonomous Campeche University. San Francisco de Campeche, Campeche, Mexico
Abstract
The main objective was to select and identify native lactic acid bacteria from the gastrointestinal tract of Nile tilapia (Oreochromis niloticus) to use as a possible probiotic. Seventeen Lactic Acid Bacteria (LAB) were isolated from the gastrointestinal tract of juvenile Nile tilapia. Six strains were selected and named in the first phenotypic and morphological selection process: C3, C4, C7, C15, C16, and C25. The strains C3, C7, and C25 presented high antagonism activity towards pathogens strain, high survival index against bile salts and acidity tolerances, optimum acid lactic production, and enzymatic activity for carbohydrates and protein substrate. Therefore, strains C3, C7, and C16 presented potential probiotics for Nile tilapia aquaculture. The biological effects of the strains in Nile tilapia will study in a future experiment where the strains will include in fish feed.
Keywords