Mycobiota and mycotoxins present in finished fish feeds from farms in the Rio de Janeiro State, Brazil

Authors

• Tatiana S Barbosa ¹
• Carina M Pereyra ^{2, 3}
• Carla A Soleiro ^{1, 4}
• Erica O Dias ¹
• Aguida A Oliveira ^{1, 4}
• Kelly M Keller ^{5, 4}
• Pedro PO Silva ¹
• Lilia R Cavaglieri ^{2, 3}
• Carlos AR Rosa ^{1, 4}

¹ Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rod. BR465 Km 7, Seropédica, Rio de Janeiro 23890-000, Brazil.

² Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto (UNRC), Ruta Nacional No. 36 Km 601, Río Cuarto, Córdoba X5804BYA, Argentina

³ Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires C1033AAJ, Argentina

⁴ Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasília 71605-001, Brazil.

⁵ Universidade Federal Rural do Rio de Janeiro (UFRRJ), Rod. BR465 Km 7, Seropédica, Rio de Janeiro 23890-000, Brazil

10.1186/2008-6970-5-3

Abstract

The aim of the present study was to determine species of the fungal genera Aspergillus, Fusarium, and Penicillium and fumonisin B1 (FB1), aflatoxin B1 (AFB1), and ochratoxin A (OTA) contamination from feed intended for fish farms. A total of 60 samples were sampled from tilapia farms in the Rio de Janeiro State, Brazil. The quantitative enumeration of fungi as colony-forming units per gram of feed (CFU/g) was performed using the surface spread method in different culture media. The results were expressed as fungal isolation frequency and relative density. Fungal total counts ranged from Fusarium counts were not observed. Among toxigenic genera, Aspergillus (68%) was the most prevalent, followed by Penicillium species (60%). Aspergillus niger aggregate (36%), Aspergillus flavus (35%), and Penicillium citrinum (71%) were the most prevalent species. A high percentage of samples (98%) were contaminated with FB1 levels, while 55% and 3.3% were contaminated with AFB1 and OTA, respectively. The simultaneous occurrence of these mycotoxins emphasizes the need for further research in the area to better assess the risk to the health of fish farms and their implications for the health of consumers of this meat.

Keywords

• Aflatoxin B1
• Fish feed
• Fumonisin B1
• Mycobiota
• Ochratoxin A

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