Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus

Authors

1 Division of Environmental Symbiosis, Graduate School of Integrated Arts and Sciences, Tokushima University, Tokushima 770-8502, Japan

2 Fukae Nagasaka Clinic, Etajima 737-2214, Japan

3 Division of Environmental Symbiosis, Graduate School of Integrated Arts and Sciences, Tokushima University

4 Junior College Division, Department of Science for Human Health, Shikoku University, Tokushima 771-1192, Japan

5 Department of Pharmacology, Osaka Dental University, Hirakata 573-1121, Japan

Abstract

A novel lectin was purified from the dorsal spines of the devil stinger, Inimicus japonicus using a combination of affinity chromatography techniques. A single band was detected on a native PAGE gel with a relative molecular mass of 97 kDa. The N-terminal partial amino acid of the intact 75 kDa subunit of the 97 kDa lectin was found to be DHEDS. The agglutination of rabbit erythrocytes by the 97 kDa lectin was inhibited most effectively by methyl α-D-mannoside. The 97 kDa lectin stimulated mitogenesis in murine splenocytes. This is the first study to examine the dorsal lectin of I. japonicus and one of the very few studies on venom lectins from venomous scorpaeniform fish. These results suggest that the devil stinger, I. japonicus, may be a novel resource for biologically active substances.

Keywords

International Aquatic Research
Volume 7, Issue 2
June 2015
Pages 143-150

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